Aseptic Enclosures, Sterile Air & Containment Equipment
By Fran McAteer, Microbiology Research Associates
Compounding sterile preparations (CSPs) are made utilizing aseptic technique. Aseptic technique is a microbiological term referring to the prevention of microorganism contamination. The procedure involves the use of specialized equipment, sterile apparel, meticulous processing, and continuous cleaning.
Aseptic technique of pharmacy operators was assessed using simulated media-fill tests challenged with microorganisms. Simulation of the process was done in accordance with multiple transfer steps using tryptone soya broth. All stoppers of broth medium vials were deliberately contaminated with a challenge micro-organism (Enterococcus faecalis). Each final preparation
Aseptic techniques used together with chlorhexidine reduced the infection rate and prevented cross-infection in patients undergoing urinary-tract catheterisation. Infection of the bladder appears to have arisen by contamination of the catheter-meatal junction, at which site antiseptic prophylaxis in the presence of bathing was ineffective. Patients having short-term catheterisation should not be placed in baths, since wetting the perineum will contaminate the urethral meatus with mixed enterobacteria, predisposing the female patient in particular to infection.
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